不同镉浓度处理下水稻植株镉含量变化及其镉调控相关基因表达分析

doi:10.16819/j.1001-7216.2016.6031

摘要/Abstract

摘要：

以地上部镉含量不同的籼稻和粳稻品种各6个为材料，采用水培试验，设置6个镉浓度，研究了不同浓度镉处理对不同水稻品种植株生长、镉含量和相关重金属转运蛋白基因表达水平的影响。结果表明，镉胁迫对水稻生长有抑制作用，表现为苗高、地上部干物质量和叶绿素含量下降。在不同镉浓度处理下，植株根部和地上部的镉含量存在显著的基因型差异，植株中镉从根部向地上部的转运可能是水稻品种间以及籼粳亚种间地上部镉含量差异的主要原因，根部镉的吸收量影响较小。在0.50到2.00 mg/kg的高镉处理梯度间，部分水稻品种地上部出现镉积累饱和现象（镉含量增加较少，部分水稻品种地上部镉含量甚至显著降低）；在此镉浓度处理下，OsNRMAP1、OsIRT2和OsNAS2的表达水平显著上调，这可能与植株对镉的吸收调节作用相关。

关键词: 水稻, 镉, 基因型, 植株生长, 基因表达

Abstract:

Using six indica and six japonica cultivars, differed in cadmium concentrations in the aboveground parts, the growth, heavy metal contents and expression levels of heavy metal transporter related genes were studied in a hydroponic experiment at six cadmium levels. Cadmium stress reduced plant height, shoot dry weight and chlorophyll content in rice leaves, inhibiting rice growth. Significant difference in cadmium content in rice roots and shoots among rice cultivars were found under different cadmium treatments, and the results showed that roottoshoot translocation may be a key process that determine the difference in cadmium contents between rice cultivars and subspecies, while cadmium absorption by roots had little effect. At various cadmium levels (from 0.50 to 2.00 mg/kg), saturation of cadmium accumulation in rice shoots were found in some cultivars that cadmium content stopped increasing or even decreased. Besides, the expression levels of OsNRMAP1，OsIRT2 and OsNAS2 were significantly increased at such cadmium treatment, which might be attributed to the regulation of rice plants.

Key words: rice, cadmium, genotype, plant growth, gene expression

中图分类号:

周全,王宏,张迎信,董青,孟帅,曹立勇,邵国胜*,沈希宏*. 不同镉浓度处理下水稻植株镉含量变化及其镉调控相关基因表达分析[J]. 中国水稻科学 2016,30(4): 380-388. , DOI: 10.16819/j.1001-7216.2016.6031 .

ZHOU Quan, WANG Hong, ZHANG Yingxin, DONG Qing, MENG Shuai, CAO Liyong, SHAO Guosheng*, SHEN Xihong*. Changes in Cadmium Concentration in Rice Plants Under Different Cadmium Levels and Expression Analysis of Genes Retated to Cadmium Regulation[J]. Chinese Journal of Rice Science 2016,30(4): 380-388., DOI: 10.16819/j.1001-7216.2016.6031 .

参考文献

[1]Zhou H, Zeng M, Zhou X, et al. Heavy metal translocation and accumulation in iron plaques and plant tissues for 32 hybrid rice (Oryza sativa L.) cultivars. Plant & Soil, 2015, 386(1/2): 317329.
[2]Kikuchi T, Okazaki M, Toyota K, et al. The inputoutput balance of cadmium in a paddy field of Tokyo. Chemosphere, 2007, 67(5): 920927.
[3]王英英, 钱蜀, 邓星亮. 成都平原西部农业土壤中金属元素分布特征研究. 三峡环境与生态, 2012, 34(5): 1114, 18.
Wang Y Y, Qian S, Deng X L. Investigation into heavy metals distribution in the farmland soils in the western of Chengdu plain. Environ Ecol Three Gorg, 2012, 34(5): 1114, 18. (in Chinese)
[4]詹杰, 魏树和, 牛荣成. 我国稻田土壤镉污染现状及安全生产新措施. 农业环境科学学报, 2012, 31(7): 12571263.
Zhan J, Wei S H,Niu R C. Advances of cadmium contaminated paddy soil research and new measure of its safe production in China: A review. J AgroEnviron Sci, 2012, 31(7): 12571263. (in Chinese with English abstract)
[5]甄燕红, 成颜君, 潘根兴, 等. 中国部分市售大米中 Cd, Zn, Se 的含量及其食物安全评价. 安全与环境学报, 2008, 8(1): 119122.
Zhen Y H, Cheng Y J, Pan G X, et al. Cd, Zn and Se contents of the polished rice samples from some Chinese open markets and their relevance to food safety. J Safe Environ, 2008, 8(1): 119122. (in Chinese with English abstract)
[6]Liu J, Zhu Q, Zhang Z, et al. Variations in cadmium accumulation among rice cultivars and types and the selection of cultivars for reducing cadmium in the diet. J Sci Food Agric, 2005, 85: 147153.
[7]Liu J, Qian M, Cai G, et al. Uptake and translocation of Cd in different rice cultivars and the relation with Cd accumulation in rice grain. J Hazard Mater, 2007, 143(1): 443447.
[8]胡培松. 土壤有毒重金属镉毒害及镉低积累型水稻筛选与改良. 中国稻米, 2004, 14(3): 165169.
Hu P S. Cadmium detoxification in soil and breeding of new rice with less cadmium accumulation. China Rice, 2004, 14(3): 165169. (in Chinese)
[9]He J, Zhu C, Ren Y, et al. Genotypic variation in grain cadmium concentration of lowland rice. J Plant Nutr Soil Sci, 2006, 169(5): 711716.
[10]Liu J, Zhu Q, Zhang Z, et al. Variations in cadmium accumulation among rice cultivars and types and the selection of cultivars for reducing cadmium in the diet. J Sci Food&Agric, 2005, 85(1): 147153.
[11]Takahashi R, Ishimaru Y, Senoura T, et al. The OsNRAMP1 iron transporter is involved in Cd accumulation in rice. J Exp Bot, 2011, 62(14): 48434850.
[12]Takahashi R, Ishimaru Y, Nakanishi H, et al. Role of the iron transporter OsNRAMP1 in cadmium uptake and accumulation in rice. Plant Signal & Behav, 2011, 6(11): 18131816.
[13]Sasaki A, Yamaji N, Yokosho K, et al. Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice. Plant Cell, 2012, 24(5): 21552167.
[14]Ishikawa S, Ishimaru Y, Igura M, et al. Ionbeam irradiation, gene identification, and markerassisted breeding in the development of lowcadmium rice. Proc Natl Acad Sci, 2012, 109(47): 1916619171.
[15]Nakanishi H, Ogawa I,Ishimaru Y, et al. Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+ transporters OsIRT1 and OsIRT2 in rice. Soil Sci &Plant Nutr, 2006, 52(4): 464469.
[16]Yamaji N, Xia J, MitaniUeno N, et al. Preferential delivery of zinc to developing tissues in rice is mediated by Ptype heavy metal ATPase OsHMA2. Plant Physiol, 2013, 162(2): 927939.
[17]Takahashi R, Ishimaru Y, Shimo H, et al. The OsHMA2 transporter is involved in roottoshoot translocation of Zn and Cd in rice. Plant, Cell & Environ, 2012, 35(11): 19481957.
[18]Miyadate H, Adachi S, Hiraizumi A, et al. OsHMA3, a P1Btype of ATPase affects roottoshoot cadmium translocation in rice by mediating efflux into vacuoles. New Phytol, 2011, 189(1): 190199.
[19]Uraguchi S, Kamiya T, Sakamoto T, et al. Lowaffinity cation transporter (OsLCT1) regulates cadmium transport into rice grains. Proc Natl Acad Sci, 2011, 108(52): 2095920964.
[20]Uraguchi S, Kamiya T, Clemens S, et al. Characterization of OsLCT1, a cadmium transporter from indica rice (Oryza sativa). Physiol Plant, 2014, 151(3): 339347.
[21]Shao G, Chen M, Wang W, et al. Iron nutrition affects cadmium accumulation and toxicity in rice plants. Plant Growth Regul, 2007, 53(1): 3342.
[22]Cheng L, Wang F, Shou H, et al. Mutation in nicotianamine aminotransferase stimulated the Fe (II) acquisition system and led to iron accumulation in rice. Plant Physiol, 2007, 145(4): 16471657.
[23]Inoue H, Higuchi K, Takahashi M, et al. Three ricenicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are expressed in cells involved in longdistance transport of iron and differentially regulated by iron. Plant J, 2003, 36(3): 366381.
[24]邵国胜, Muhammad J H, 章秀福, 等. 镉胁迫对不同水稻基因型植株生长和抗氧化酶系统的影响. 中国水稻科学, 2004, 18(3): 239244.
Shao G S, Muhammad J H, Zhang X F, et al. Effects of cadmium stress on plant growth and antioxidative enzyme system in different rice genotypes. Chin J Rice Sci, 2004, 18(3): 239244. (in Chinese with English abstract)
[25]何俊瑜, 任艳芳, 朱诚, 等. 镉胁迫对不同水稻品种种子萌发, 幼苗生长和淀粉酶活性的影响. 中国水稻科学, 2008, 22(4): 399404.
He J Y, Ren Y F, Zhu C, et al. Effects of cadmium stress on seed germination, seedling growth, and amylase activities in rice. Chin J Rice Sci, 2008, 22(4): 399404. (in Chinese with English abstract)
[26]覃都, 陈铭学, 周蓉, 等. 锰镉互作对水稻生长和植株镉, 锰含量的影响. 中国水稻科学, 2010, 24(2): 189195.
Qin D, Chen M X, Zhou R, et al.Effects of interaction between manganese and cadmium on plant growth and contents of cadmium and manganese in rice. Chin J Rice Sci, 2010, 24(2): 189195. (in Chinese with English abstract)
[27]Lee S, Persson D P, Hansen T H, et al. Bioavailable zinc in rice seeds is increased by activation tagging of nicotianamine synthase. Plant Biotechnol J, 2011, 9(8): 865873.
[28]Das P, Samantaray S, Rout G R. Studies on cadmium toxicity in plants: A review. Environ Pollut, 1997, 98(1): 2936.
[29]Hart J J, Welch R M, Norvell W A, et al. Characterization of cadmium binding, uptake, and translocation in intact seedlings of bread and durum wheat cultivars. Plant Physiol, 1998, 116(4): 14131420.
[30]Hart J J, Welch R M, Norvell W A, et al. Characterization of cadmium uptake, translocation and storage in near‐isogenic lines of durum wheat that differ in grain cadmium concentration. New Phytol, 2006, 172(2): 261271.
[31]Zhao F J,Hamon R E, Lombi E, et al. Characteristics of cadmium uptake in two contrasting ecotypes of the hyperaccumulator Thlaspi caerulescens. J Exp Bot, 2002, 53(368): 535543.
[32]Chan D Y, Hale B A. Differential accumulation of Cd in durum wheat cultivars: Uptake and retranslocation as sources of variation. J Exp Bot, 2004, 55(408): 25712579.
[33]Uraguchi S, Mori S, Kuramata M, et al. Roottoshoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice. J Exp Bot, 2009, 60(9): 26772688.
[34]Zhao F J, McGrath S P, Meharg A A. Arsenic as a food chain contaminant: mechanisms of plant uptake and metabolism and mitigation strategies. Ann Rev Plant Biol, 2010, 61: 535559.